fbpx

How Microbes Break Down Wastewater Contaminants

09.15.2015

Researchers conducted analyses of microbial communities in laboratory-scale bioreactors breaking down contaminated wastewater from gold ore processing.

In this "first application of genome-resolved metagenomics" to characterize bioreactors involved in gold ore processing, the data reveal how the bioreactors utilize available nutrients in wastewater and allow researchers to observe which microbes dominate the communities, which will help improve microbial-based remediation strategies.

The Emerging Technologies Opportunity Program (ETOP) was launched in 2013 by the U.S. Dept. of Energy Joint Genome Institute (DOE JGI), a DOE Office of Science User Facility at Lawrence Berkeley National Laboratory. The Program aims to bring new technologies developed at other institutions into the DOE JGI, making them available to its users for energy and environment applications. A half a dozen proposals were approved during the Program's first year.

Among the projects approved in the inaugural ETOP call was one from metagenomics pioneer Jill Banfield at the Univ. of California, Berkeley. Banfield proposed building and characterizing a pipeline that would allow researchers to isolate and study both near-complete and complete microbial genomes from environmental samples. One of the results from this project was published in Environmental Microbiology.

Natural ecosystems and the diverse communities of microbes (and other organisms) within them are extremely difficult to study because they are not closed systems, where all inputs and outputs can be accessed. To make the compositions and activities of microbial communities more tractable for analyses with genomic methods, this study focused on microbial communities in laboratory bioreactors that were being studied as a potential method of treating wastewater contaminated by gold ore processing. From these bioreactors, the team reconstructed draft and curated microbial genomes using high-throughput metagenomic sequencing of biofilm and supernatant samples. In one bioreactor, a mixture of cyanide (CN-)—used for processing gold ore—and thiocyanate (SCN-)—a byproduct of the process—was being degraded, while in the other bioreactor, only thiocyanate was being degraded.

"This is the first application of genome-resolved metagenomics to characterize SCN− and CN− bioreactors," the team noted, "revealing a complex community containing novel organisms and genes." The analyses allowed the team to outline the structures of the microbial communities and diagram potential nutrient flow paths. For example, they found evidence indicating that the microbes were not relying on the molasses included in the media as an energy source, which could help reduce bioreactor operating costs on the commercial scale. They also recovered several genome sequences allowing them to determine the composition of the communities in the bioreactors. They found some functions are shared, such as the ability to adapt to temperature and oxygen fluctuations. Others are less so; they found a complete denitrification pathway in one microbial species in the CN-SCN processing bioreactor.

Ultimately, this study will provide new approaches for the scientific community towards characterizing microbial communities involved in activities of major interest to DOE, including support of bioenergy feedstock plants, terrestrial carbon cycling, and waste cleanup.

Article cited from: http://goo.gl/fYzYYv

 

News/Events 

  1. Waste No Waste: Time to Embrace Biogas
  2. Is Big Gas finally learning to love biogas?
  3. We need to get behind Renewable Natural Gas
  4. Difference between a Turbo and Positive Displacement Blower
  5. The Difference between Methane and Natural Gas
  6. First Dairy Biogas Project in Connecticut
  7. Does Renewable Natural Gas Have a Future in Energy?
  8. Biogas Offtake Opportunities For Digesters
  9. Wisconsin Dairy Begins Production of Renewable Natural Gas
  10. Anaerobic Digestion Sector Forming a Clearer Picture
  11. Brightmark to Expand Western New York Dairy Biogas Project
  12. Biogas - The Energy Wonder That's Under Our Noses
  13. Power Generation Achieved by a Self-Assembled Biofuel Cell
  14. Less Carbon Dioxide from Natural Gas
  15. Project Uses Renewable Electricity for RNG Production
  16. Smithfield Hog Farm Provides Natural Gas to Missouri City
  17. From Waste to Gas
  18. Gas Clash Threatens Australian Export
  19. Maximizing Opportunities of Anaerobic Digestion from Wastewater
  20. Catalyst to Speed up Conversion of Biomass to Biofuel
  21. How It Works: Ethanol
  22. Anaerobic Digestion - the Next Big Renewable Energy Source
  23. Anaerobic Additions
  24. Three (3) Tech Solutions for Modern Landfills
  25. The Costs and Benefits of Anaerobic Digesters
  26. Bacteria Farts Power Wastewater Plant in Fort Wayne
  27. Europe’s First Poultry Manure Biogas Plant
  28. Electricity Using Pig Manure
  29. $38-Million Biodigester coming to Grand Rapids
  30. Biochar Could Benefit Anaerobic Digestion of Animal Manure

For additonal reading, please visit us at: News Worthy

Difference between a Turbo and Positive Displacement Blower